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CAS RN: 28718-90-3 | Product Number: D5888

DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]


Purity:
Synonyms:
  • 4',6-Diamidino-2-phenylindole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
  • 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride (0.2mL×5) (1mg/mL in Water)
  • 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
Product Documents:
1SET
$40.00
25   3  

* Please contact our distributors or TCI to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.


Supplemental Product Information:

Product Information

  • Volume : 0.2 mL x 5 vial
  • Concentration : 1.0 mg/mL

Description

  • The DAPI is a fluorescent stain for labeling DNA.
    DNA-bound DAPI is readily excited with UV light and emits fluorescence in the blue region of the visible light spectrum (Excitation : 358 nm / Emission : 461 nm).

Description for Use

  • Fluorescence Staining of Cell Nucleus
    1. Fix and permeabilize cells as desired.
    2. Dilute DAPI solution 1/1000 (*) with PBS or an appropriate buffer.
      *Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL.
    3. Incubate with cells at room temperature for 5-20 minutes (**).
      **Extend Incubate time as desired.
    4. Mount sample with an appropriate medium and proceed to imaging.

Storage

  • Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Related Products

  • A2412 DAPI·2HCl [for Biochemical Research]
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  • E1363 Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
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  • G0569 Goat Anti-Mouse IgG R-PE Conjugate
  • G0577 Goat Anti-Rabbit IgG R-PE Conjugate
  • H1343 Bisbenzimide H 33258 Hydrate [for Biochemical Research]
  • S0966 Streptavidin FITC Conjugate
  • T3885 Streptavidin R-PE Conjugate

Product Number D5888
Molecular Formula / Molecular Weight C__1__6H__1__5N__5·2HCl = 350.25 
Physical State (20 deg.C) Liquid
Storage Temperature Frozen (<0°C)
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 28718-90-3
Reaxys Registry Number 4894417
PubChem Substance ID 468591628
MDL Number

MFCD00012681

Specifications
Appearance Light yellow to Yellow clear liquid
DNA staining test pass test
Properties (reference)
Maximum Absorption Wavelength 342(H2O) nm
GHS
Related Laws:
RTECS# NL5995054
Transport Information:
H.S.code* 2933.99-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
Application
Fluorescent staining of DNA in cells

References


TCIMAIL
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