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CAS RN: | 产品编码: T3531
Streptavidin Maleimide Conjugate
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Product Information
Volume/Quantity:0.5 mg
Conjugation:Purified streptavidin was conjugated to Sulfo-SMCC
(TCI : code S0883).
Product Form:Streptavidin Maleimide Conjugate.
Lyophilized in 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2.
Preservative:None
Specific Activity:>10 U/mg , HABA dye-binding method
Description
Streptavidin Maleimide Conjugate is designed to facilitate the conjugation of the Streptavidin to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups.
Protocol
1. Allow the vial of Streptavidin Maleimide Conjugate to warm to room temperature.
2. Reconstitute the Streptavidin Maleimide Conjugate by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
Note: The buffer in which the thiol-containing molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA (1-10 mM) may help to prevent metal catalysed oxidation of thiols.
3. React for 30–60 minutes at 37 ºC or 2 hours at room temperature.
4. If desired, desalt, dialyze or purify the conjugate.
Storage
Store at －20 ºC to －80 ºC (Preferably at －80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
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| 产品编码 | T3531 |
| 外观与形状（20°C） | 固体 |
| 储存温度 | 冷冻 (-80°C) |
| 应避免的情况 | 加热 |
| PubChem物质ID | 354335029 |
技术规格
| Appearance | White to Brown to Dark green powder to crystal |
| Specific Activity | to pass test |
物性（参考值）
GHS
相关法规
运输信息
| HS编码* | 3504.00-000 |
应用
Streptavidin Maleimide Conjugate
References
- Spectrophotometric determination of avidin and biotin.
- Isolation and characterization of highly purified streptavidin obtained in a two-step purification procedure from Streptomyces avidinii grown in a synthetic medium.
- G. T. Hermanson, in Bioconjugate Techniques, 2nd ed., Academic Press, 2008, pp.905-909.
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