RIPA Buffer (Ready-to-use) [for Protein extraction]

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纯度/分析方法:

别名：

• RIPA 缓冲液 (即用型) [用于蛋白质提取]

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补充产品信息

规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
100ML	S$118.50	23	8	请联系我们		货期预估

* 订购TCI产品，请联系我们的经销商或 联系我们。
* TCI会时常优化储存条件，敬请留意。

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大包装询价

Product Information

• Volume : 100 mL
• Additives : WITHOUT protease inhibitors

Description

• This product is supplied as a ready-to-use solution to lyse cultured mammalian cells. The extraction from cultured cells by RIPA buffer (Product No. R0246) can be transferred directly to further analysis. This product does not include protease inhibitors, thus please add protease inhibitor cocktail to the product as necessary.

Direction for Use: Lysis of adherent cells

• 1. Remove medium from cultured cells and wash cells twice with cold PBS.
  2. Remove PBS and add cold RIPA buffer (Product No. R0246) to the cells. 1 mL of RIPA buffer is added to 0.5 - 5.0 x 10⁷ cells.
  3. Scrape the adherent cells with a cell scraper and transfer the lysate and pellet to a tube.
  4. Incubate the cells for 15 minutes on ice. (To increase the yields, prolong the incubation.)
  5. Centrifuge the tube at 10,000 x g for 10 minutes at 4 °C.
  6. Transfer the supernatant to a new tube for further analysis.

Direction for Use: Lysis of suspension cells

• 1. Transfer the cultured cells to a tube, remove medium from cultured cells and wash cells twice with cold PBS.
  2. Remove PBS and add cold RIPA buffer (Product No. R0246) to the cells. 1 mL of RIPA buffer is added to 0.5 - 5.0 x 10⁷ cells.
  3. Incubate the cells for 15 minutes on ice. (To increase the yields, prolong the incubation.)
  4. Centrifuge the tube at 10,000 x g for 10 minutes at 4 °C.
  5. Transfer the supernatant to a new tube for further analysis.

Storage

• The product should be stored at 4 °C and used within 6 months.

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