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CAS RN: | 产品编码: B6105
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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| 规格 | 单价 | 同一天 | 2-3个工作日 | 数量 |
|---|---|---|---|---|
| 10ML |
S$75.00
|
≥60 | 10 |
|
补充产品信息:
Product Information
Volume : 10 mLDescription
This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25 - 30 °C if precipitation occurs during storage.
Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95 - 100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (TCI Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (TCI Product No. D3647), etc.
Storage
Store at room temperature.
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B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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M1948 2-Mercaptoethanol [for Electrophoresis]
| 产品编码 | B6105 |
| 外观与形状（20°C） | 液体 |
| 储存温度 | 室温 (15°C以下阴凉干燥处) |
技术规格
| Appearance | Green to Dark green to Dark blue clear liquid to cloudy liquid |
| Functionality test | to pass test(Electrophoresis) |
物性（参考值）
| 比重 | 1.16 |
GHS
| 象形图 |
|
| 信号词 | Warning |
| 危险性说明 | H315 : Causes skin irritation. |
| 防范说明 | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves. P302 + P352 : IF ON SKIN: Wash with plenty of water. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
相关法规
运输信息
| HS编码* | 3822.19-000 |
应用
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
References
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
- Dodecyl sulfate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.
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