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CAS RN: | 产品编码: A2659
AOL-Biotin Conjugate
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Product Information
Quantity : 1 ml per vial (1 mg/1 ml: Lowry method)Product Form : Biotin-immobilized recombinant AOL, Liquid
Buffer : 50 mM Phosphate buffer, pH6.5
Molecular Mass : >35 kDa
Preservative : None
Stabilizer : None
Description
The AOL is a carbohydrate-binding protein (Lectin) derived from Aspergillus oryzae. The AOL gene product is expressed in Aspergillus oryzae. (The gene is derived from the same origin as the host cell.) This lectin preferably binds to a [Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)] glycan epitopes.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The AOL-Biotin Conjugate shows significant interaction with human lactoferrin (hLF) possessing [Fucα(1-6)/α(1-3)] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the AOL-Biotin Conjugate specifically binds to α(1-6/1-3)Fucsylated hLF but not to unfucosylated glycoproteins such as human transferrin (hTF), asialo hTF, agalacto hTF and bovine RNase B.
Storage
Store at 2-10°C.
Related Products
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5 mg/ml, PBS pH6.5)A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
| 产品编码 | A2659 |
| 外观与形状（20°C） | 液体 |
| 储存温度 | 冷藏 (0-10°C) |
| 应避免的情况 | 加热 |
技术规格
| Appearance | Colorless to Light yellow clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 30 to 40 kDa |
| Suitability for use in ELISA tests | min. 1.0 (human Lactoferrin, 10 micro g/mL, OD450) |
| Fucose inhibition test | to pass test |
物性（参考值）
GHS
相关法规
运输信息
| HS编码* | 3504.00-000 |
应用
Fucose-specific lectin
References
- Molecular cloning and overexpression of fleA gene encoding a fucose-specific lectin of Aspergillus oryzae
- Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose
- Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin
应用
Biotinylated Fucose-specific Lectin
References
- Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae
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