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CAS RN: | Product Number: L0466
Linear PEI HCl [1mg/mL in PBS(-)] [for Transfection]
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Purity:
Synonyms:
- Linear Polyethyleneimine Hydrochloride [1mg/mL in PBS(-)]
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time | Quantity |
Shipping Information
|
|---|---|---|---|---|---|---|
| 1ML |
S$42.00
|
≥40 | 14 | Contact Us |
|
|
| 10ML |
S$286.50
|
21 | 0 | Contact Us |
|
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* The storage conditions are subject to change without notice.
Supplemental Product Information:
Description
- This product is supplied as a pH-adjusted, 1 mg/mL stock solution of the linear form of the cationic polymer polyethylenimine (PEI) to be used for the transfection of nucleic acids into living cells.
Direction for Use
-
- Prepare two aliquots of OPTI-MEM®.
Each should be equivalent to 1/4 the regular culture maintenance volume of your cells. - Into one aliquot, mix an appropriate amount of Linear PEI HCl (Product No. L0466) (*).
- Into the other aliquot, mix an appropriate amount of DNA (*).
- Combine the two aliquots from step 2 and incubate at 37 °C for 20 minutes.
- Add an equivalent volume of regular culture medium to the solution from step 3.
- Aspirate medium from cells and replace with the medium from step 4.
- Allow transfection to proceed for between 6 and 24 hours.
- Replace medium and incubate until protein expression is detected.
- Cells can now be used in downstream applications.
- Prepare two aliquots of OPTI-MEM®.
- * Before running any transfection experiments, optimal transfection conditions (PEI concentration, N:P ratio) for your specific cell line must be determined. We recommend starting with final PEI concentrations ranging from 1 to 6 µg/mL, and N:P ratios of between 10 and 30.
Storage
- Store at 4 °C. Use within 2 years of receipt.
| Product Number | L0466 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Transfection Efficiency Test | to pass test |
| Cytotoxicity Test | to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
A Ready-to-Use Cationic Polymer for Gene Transfection
References
- 1) Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery
- 2) Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethylenimine-mediated DNA delivery
- 3) Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung
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