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CAS RN: | Product Number: G0452
Goat Anti-Rabbit IgG FITC Conjugate
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Product Information
Volume/Quantity:0.1 mg
Product Form:Purified goat polyclonal antibody/IgG, Fluorescein 5-Isothiocyanate (FITC) conjugated
Antibody Preparation:Antiserum was prepared by injection of purified whole rabbit IgG into healthy goat without clinical history of infectious disease.
Conjugation:Affinity purified antibody was conjugated to FITC (Ex:494 nm, Em:520 nm)
Preservative:0.05% Sodium azide
Immunogen:Whole rabbit IgG
Specificity:Rabbit IgG
Applications
This antibody reacts with rabbit IgG on FLISA (1:400).
Storage
Store at 4ºC for 1 week or －20ºC for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Related Products
G0388 Goat Anti-Rabbit IgG
G0389 Goat Anti-Rabbit IgG Biotin Conjugate
G0418 Goat Anti-Rabbit IgG HRP Conjugate
F0026 Fluorescein 5-Isothiocyanate (isomer I)
| Product Number | G0452 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Light yellow to Amber to Dark green clear liquid |
| Titer(ELISA) | to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| H.S.code* | 3504.00-000 |
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
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