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CAS RN: | Product Number: G0452
Goat Anti-Rabbit IgG FITC Conjugate
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Supplemental Product Information:
Product Information
- Volume/Quantity: 0.1 mg
- Product Form: Purified goat polyclonal antibody/IgG, Fluorescein 5-
Isothiocyanate (FITC) conjugated - Antibody Preparation: Antiserum was prepared by injection of purified whole rabbit IgG into healthy goat without clinical history of infectious disease.
- Conjugation: Affinity purified antibody was conjugated to FITC
(Ex:494 nm, Em:520 nm) - Preservative: 0.05% Sodium azide
- Immunogen: Whole rabbit IgG
- Specificity: Rabbit IgG
Applications
- This antibody reacts with rabbit IgG on FLISA (1:400).
Storage
- Store at −20 ºC to −80 ºC (Preferably at −80 ºC). Storage at 4 ºC is not
recommended. Avoid repeated freezing and thawing. This product is
photosensitive and should be protected from light.
Related Products
- G0388 Goat Anti-Rabbit IgG
- G0597 Goat Anti-Rabbit IgG Biotin Conjugate
- G0418 Goat Anti-Rabbit IgG HRP Conjugate
- F0026 Fluorescein 5-Isothiocyanate (isomer I)
| Product Number | G0452 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Yellow to Orange to Green clear liquid to cloudy liquid |
| Titer(ELISA) | to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
| H.S.code* | 3504.00-000 |
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
Documents
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Specifications
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Other Documents
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