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CAS RN: 131467-87-3 | Product Number: A5557
NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling]
Purity: >98.0%(HPLC)
Synonyms:
- 4-Hydrazino-7-nitrobenzofurazan Hydrazine
- 4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine
Product Documents:
Size | Unit Price | Same Day | 2-3 Business Days |
---|---|---|---|
100MG |
$213.00
|
10 | ≥100 |
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* The storage conditions are subject to change without notice.
Product Number | A5557 |
Purity / Analysis Method | >98.0%(HPLC) |
Molecular Formula / Molecular Weight | C__6H__5N__5O__3·H__4N__2 = 227.18 |
Physical State (20 deg.C) | Solid |
Storage Temperature | Refrigerated (0-10°C) |
Store Under Inert Gas | Store under inert gas |
Condition to Avoid | Light Sensitive,Air Sensitive,Heat Sensitive |
Packaging and Container | 100MG-Glass Bottle with Plastic Insert (View image) |
CAS RN | 131467-87-3 |
PubChem Substance ID | 87563175 |
MDL Number | MFCD00191508 |
Specifications
Appearance | Orange to Brown powder to crystal |
Purity(HPLC) | min. 98.0 area% |
Effect test of HPLC derivatization | Effective as labeling agent for Propionaldehyde |
NMR | confirm to structure |
Properties (reference)
GHS
Pictogram | |
Signal Word | Warning |
Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
Related Laws:
Transport Information:
H.S.code* | 2934.99-000 |
Application
HPLC Labeling Reagent for Carbonyl Compounds
The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carbonyl group to form the corresponding hydrazone. The labeling reagent itself is non-fluorescent, but the hydrazones after the reaction with carbonyl compounds have strong fluorescence. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 550 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants, and a highly sensitive detection can be done because of its high reactivity.
Application examples:
[Aldehydes or ketones] 1)
250 µM of the labeling reagent 1 and 1.7 µM propionaldehyde are added to acetonitrile containing 0.025% TFA, and reacted at room temperature for 1 h, use it as the HPLC sample.
For example, the detection limit for propionaldehyde is 35 fmol.
[Aldehydes or ketones] 1)
250 µM of the labeling reagent 1 and 1.7 µM propionaldehyde are added to acetonitrile containing 0.025% TFA, and reacted at room temperature for 1 h, use it as the HPLC sample.
For example, the detection limit for propionaldehyde is 35 fmol.
TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )
HPLC Labeling Reagents ( PDF 10MB )
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