Fluorescent Label

Fluorescent label, N-succinimidyl 7-methoxycoumarin-3-carboxylate (1), has been applied to labeling not only for macromolecules such as proteins, but also for small molecules. One of the examples is introduced below.

It has been proposed that monitoring of biofluid viscosity could provide a diagnostic tool for the detection of diseases.¹⁾ Recently, a procedure for the synthesis of a ratiometric viscosity fluorescent sensor using 1 has been reported.²⁾ In this report, the coumarin moiety was used as a viscosity-independent fluorophore, and p-aminocinnamonitrile dye as a viscosity-dependent fluorophore.