Fluorescent Probe for Drugs-Human Serum Albumin (HSA) Binding Assay
―Powerful tool for drug discovery, R&D and metabolic toxicity assay―

Investigating whether drugs bind to Human Serum Albumin (HSA) and identifying the drug binding site on HSA are important for pharmacokinetics. BD140 (Product No. D4898), developed by Chang et al., is a novel fluorescent probe which binds to HSA drug binding site 2 specifically.¹⁾ BD140 is a useful tool to check whether drugs bind to HSA drug binding site 2.

Binding of BD140 to HSA was not inhibited completely or nearly by warfarin and phenylbutazone, which bind to HSA drug binding site 1, in our result. On the other hand, binding of BD140 to HSA was inhibited sufficiently by ibuprofen, flurbiprofen and triiodobenzoic acid, which bind to drug binding site 2. Thus, BD140 is available for checking whether drugs bind to HSA drug binding site 2.