Ready-to-use Solution for Pyrogallol Red-Molybdate Protein Assay

(1) Prepare standard protein solutions with a series of dilutions.
(2) Mix P2575 with unknown protein samples, standard protein solutions and distilled water according to Table 1.
(3) Incubate for 30 minutes at room temperature.
(4) Measure absorbance at 600 nm.
(5) Prepare a standard curve by plotting the absorbance data measured in #4) after subtracting from blank absorbance (distilled water), and calculate the amount of protein in test samples.

(1) Prepare four dilution series of standard protein solutions from the concentration at 1000 µg/mL by doubling dilution.
(2) Mix 200 µL of P2575 with 10 µL each of a protein sample at an unknownconcentration, the standard protein solution and distilled water in a 96 microplate.
(3) Incubate for 30 minutes at room temperature, measure absorbance at 600 nm, and prepare a standard curve.

Substances at the following concentrations in the sample solutions do not affect the reaction results.