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Published TCIMAIL newest issue No.197
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Anti-Glycosaminoglycan Antibodies
The extracellular matrix (ECM) is an essential element for higher organisms to form cells, tissues, and organs; to control cell-cell connections and functions. The ECM also greatly affects several biological phe- nomena (such as development, aging, inflammation, wound healing, and immunity). Glycosaminoglycans (GAG), such as chondroitin sulfate, hyaluronic acid and keratan sulfate, are major components of the ECM and play an important role. Analysis of glycosaminoglycans is very difficult, especially when performing in situ analysis of cells and tissues. Thus, antibodies are particularly important as a detection tool.
Anti-chondroitin sulfate antibody can be utilized for detection of the chondroitin sulfate A or D
These chondroitin sulfate were coated on ELISA plate. These antigens and anti-chondroitin sulfate antibodies were reacted at appropriate time, then 1st antibodies were detected using appropriate secondary antibodies.
References
Anti-Keratan Sulfate Monoclonal Antibody (R-10G) [A2968]
- 1)A novel antibody for human-induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures.
- 2)A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
- 3)Characterization of glycoproteins expressing the blood group H type 1 epitope on human induced pluripotent stem (hiPS) cells.
- 4)Binding specificity of R-10G and TRA-1-60/81, and substrate specificity of keratanase II studied with chemically synthesized oligosaccharides.
Anti-Chondroitin Sulfate D Monoclonal Antibody (MO-225) [A2872]
- 1)A Monoclonal Antibody That Specifically Recognizes a Glucuronic Acid 2-Sulfate-containing Determinant in Intact Chondroitin Sulfate Chain.
- 2)Distribution of chondroitin sulphate proteoglycans and peanut agglutinin-binding molecules during bovine fetal palatine ridge formation.
- 3)Structural characterization of the epitopes of the monoclonal antibodies 473HD, CS-56,and MO-225 specific for chondroitin sulfate D-type using the oligosaccharide library.
Anti-Chondroitin Sulfate A Monoclonal Antibody (LY111) [A3143]
- 1)Occurrence of collagen and proteoglycan forms of type IX collagen in chick embryo cartilage. Production and characterization of a collagen form-specific antibody.
- 2)Structural determination of novel sulfated octasaccharides isolated from chondroitin sulfate of shark cartilage and their application for characterizing monoclonal antibody epitopes.
- 3)Construction of a chondroitin sulfate library with defined structures and analysis of molecular interactions.
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