Published TCIMAIL newest issue No.196
Maximum quantity allowed is 999
CAS RN: | Producten #: R0228
Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
Zuiverheid:
Afmeting | Eenheidsprijs | België | Japan* | Hoeveelheid |
---|---|---|---|---|
1ML |
146,00 €
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*Stock beschikbaar uit voorraad in België leverbaar in 1 tot 3 dagen
*stock beschikbaar uit voorraad in Japan leverbaar in 1 tot 2 weken (met uitzondering van gereguleerde producten en zendingen met droog ijs)
Aanvullende productinformatie:
[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product Information
Product code: R0228
Product name: Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 16 kDa
Preservative : None
Stabilizer: None
Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites. Consequently, rSRL exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0225 Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5mg/mL, PBS pH6.5)
Artikel # | R0228 |
Fysieke toestand (20 graden C) | Liquid |
Opslag condities | Frozen (-20°C) |
Te vermijden condities | Heat Sensitive |
Appearance | Colorless to Light yellow to Light orange clear liquid |
Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
Molecular weight | 10000 to 25000(SDS-PAGE) |
Hemagglutination activity | max. 12.5 micro g/mL(pH7.2) |
HS-NR (invoer / uitvoer) (TCI-E) | 3504009000 |
References
- A newly isolated lectin from the plant pathogenic fungus Sclerotium rolfsii: purification, characterization and role in mycoparasitism
- Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii
- Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin
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