DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]

The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and an amino group, easily reacts with a　carboxyl group to form the corresponding amide in the presence of a condensing agent. The resultant amide is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 450 nm and 560 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference from contaminants. Short-chain fatty acids are detectable and determinable reproducibly with a detection limit on the order of fmol.　A highly sensitive detection can be done by using chemiluminescence.

Application example:²⁾
Add 50 µL of mixed fatty acid / diethyl ether solution, 50 µL of 50 mM labeling reagent 1 / acetonitrile solution, 50 µL of triphenylphosphine / acetonitrile solution and 50 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a vessel. This mixture is kept in the dark at room temperature. Dilute this reactant mixture 100x by acetonitrile, and use 10 µL of this diluted solution as an HPLC sample solution.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )