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Published TCIMAIL newest issue No.197
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CAS RN: | Product Number: T4031
Clearing Reagent iCBiofilm-H1 [for Biofilm]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 8ML |
€90.00
|
Contact Us | 26 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
Size : 8 mLDescription
Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms. Clearing Reagent iCBiofilm-H1 has been developed to allow for the observation of biofilm structure at thicknesses of more than several tens of micrometers. Simple addition of iCBiofilm-H1 solution to a biofilm renders it transparent within several seconds, allowing for easy observation using light microscopy techniques.
*This product was developed by Associate Prof. Sugimoto of the Jikei University School of Medicine.
Storage
Store at room temperature. Use within 6 months.
Directions for Use
- Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
- After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
- Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
- Stain using appropriate fluorescence reagents.
- Remove staining reagents and add 500 µL - 1 mL of Clearing Reagent iCBiofilm-H1 (Product No.T4031).
- Observe biofilm using optical microscopy.
Related Products
T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]G0068 Glutaraldehyde (ca. 50% in Water, ca. 5.6mol/L)
| Product Number | T4031 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Functionality test | to pass test |
| Refractive index | 1.3950 to 1.4050 |
Properties (reference)
GHS
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Transport Information:
| HS Number | 3822190090 |
Application
Clearing of biofilm
Reference
- Instantaneous Clearing of Biofilm (iCBiofilm): an optical approach to revisit bacterial and fungal biofilm imaging
Application
Microscopic observation of biofilm
References
- Light and Electron Microscopic Studies of Microorganisms Growing in Rotating Biological Contactor Biofilms
Articles/Brochures
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