4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]

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Synonyms:

• p-Nitrophenyl Phosphate (Ready-to-use solution)
• pNPP (Ready-to-use solution)

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Supplemental Product Information

Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
100ML	$100.00	9	0	Contact Us	Shipping Simulation

* Please contact our distributors or TCI to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.

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Product Information
Volume : 100 mL
Preservative : 0.02% Sodium azide

Description
This product is supplied as a ready-to-use solution containing 4-nitrophenyl phosphate (pNPP) for ELISA. When this product is reacted with alkaline phosphatase, a yellow colored soluble reaction product appears. After terminating the reaction by adding NaOH solution, the absorbance of reaction product is measured at 405 nm.

Application
The following steps are for a 96-well plate format.
1. Bring pNPP solution (Product No. N1109) to room temperature.
2. Gently mix the pNPP solution (Product No. N1109).
3. Add 100uL of pNPP solution (Product No. N1109) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100 uL of 1 N NaOH (Product No. S0542) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 405 nm.

Storage
The product should be stored at 4 ºC and used within 6 months from purchase.The product may be frozen but this does not affect its quality; thaw it at room temperature before use.

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