Tissue-Clearing Reagent iTOMEI-D [for Plants]

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Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*	Quantity
5ML	$29.00	1	Contact Us	12
25ML	$86.00	2	1	≥60

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"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.

Please see a brochure (PDF) of how to use.

Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.

Storage

The product should be stored at 4 °C.

Directions for Use

Required reagents;

• Paraformaldehyde (PFA)
• PBS
• Stains* (option)
• Mounting solution**

==Process outline (All operations are performed at Room temperature)===============

• Tissue fixation
  Reagent : 1% PFA in PBS
  Time : 1hr
• PBS Wash ×3
• Decoloring
  Reagent : iTOMEI-D (TCI Product No. T3940)
  Time : 24hr
• PBS Wash ×3
• (Optional process Staining*, PBS Wash ×3)
• Clearing (RI matching)
  Reagent : Mounting solution**
  Time : Operation time is determined by the mounting solution.
• Mounting
  Reagent : Mounting solution**

* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.

** Mounting solution : Please use the suitable mounting solution for objective lens.

Related Products

P0018　Paraformaldehyde
A2412　DAPI·2HCl
E0197　Evans Blue
A0132　Acridine Orange
A0321　Tris-Base
G0316　Glycerol [for Electrophoresis]
S0111　D-(+)-Sucrose
T3530　Tissue-Clearing Reagent TOMEI [for Plants]