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CAS RN: | Product Number: C4087

Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]

× Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]

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SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications

Supplemental Product Information
1KIT
$171.00
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Supplemental Product Information:

Product Information

  • Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
  • Volume : 1 kit (for 2000 cm2 of membrane)

Description

  • This product is a reagent for the chemiluminescent detection of HRP-labeled probes that react with proteins transferred to the membrane by Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. It allows the detection of proteins at the nanogram level.

Storage

  • Store at 4 °C, protected from light. Use within 6 months.

Direction for Use

    1. Bring Solution A and Solution B to room temperature.
    2. Prepare a blotting membrane covered with HRP-conjugated probe.
    3. Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
    4. Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm2 membrane.
    5. Place the membrane with the protein blotted side up on the plastic wrap or polyethylene sheet.
    6. Pour the mixture over the surface of the membrane.
    7. Incubate the membrane with this product for 60 seconds at room temperature.
    8. Remove excess mixture using paper.
    9. Place the membrane in a new wrap or polyethylene sheet.
    10. Image the membrane using an imaging system or X-ray film.

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  • N1113 Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
  • T3855 TMB [for Western blotting] (Ready-to-use solution)

Product Number C4087
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Light Sensitive,Heat Sensitive
Specifications
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H317 : May cause an allergic skin reaction.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P261 : Avoid breathing mist or vapors.
P280 : Wear protective gloves.
P272 : Contaminated work clothing must not be allowed out of the workplace.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P333 + P313 : If skin irritation or rash occurs: Get medical advice/ attention.
P363 : Wash contaminated clothing before reuse.
Related Laws:
Transport Information:
HS Number 3822.19.0080
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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