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CAS RN: 254973-02-9 | Product Number: A5576
AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]
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![AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]")
Purity: >95.0%(HPLC)
Synonyms:
- 4-Acetamido-7-mercaptobenzofurazan
- 4-Acetamido-7-mercapto-2,1,3-benzoxadiazole
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 100MG |
$270.00
|
3 | 2 | ≥100 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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| Product Number | A5576 |
| Purity / Analysis Method | >95.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__8H__7N__3O__2S = 209.22 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | Refrigerated (0-10°C) |
| Store Under Inert Gas | Store under inert gas |
| Condition to Avoid | Air Sensitive,Heat Sensitive |
| Packaging and Container | 100MG-Glass Bottle with Plastic Insert (View image) |
| CAS RN | 254973-02-9 |
| PubChem Substance ID | 87563193 |
| MDL Number | MFCD04038408 |
Specifications
| Appearance | Light yellow to Brown powder to crystal |
| Purity(HPLC) | min. 95.0 area% |
| Effect test of HPLC derivatization | Effective as labeling agent for Caproic Acid, Lauric Acid, Myristic Acid, Palmitic Acid |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 2934.99.9001 |
Application
HPLC Labeling Reagent for Carboxylic Acids
The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a mercapto group, easily reacts with a carboxyl group to form the corresponding thioester. 1 itself fluoresces very little, but the thioester derivatives fluoresce highly. The resultant thioester is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 368 nm and 524 nm, respectively. [The detection limit = 10~20 fmol (S/N = 3)]
Application example:
Add 20 µL of mixed fatty acid / acetonitrile solution, 20 µL of 20 mM labeling reagent 1 / dichloromethane solution, 20 µL of triphenylphosphine / acetonitrile solution and 20 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a 500 µL vessel, and the mixture is left at room temperature for 15 min. Dilute this reactant mixture with 20 µL of acetonitrile, and use 1 µL of this diluted solution as an HPLC sample solution.
Add 20 µL of mixed fatty acid / acetonitrile solution, 20 µL of 20 mM labeling reagent 1 / dichloromethane solution, 20 µL of triphenylphosphine / acetonitrile solution and 20 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a 500 µL vessel, and the mixture is left at room temperature for 15 min. Dilute this reactant mixture with 20 µL of acetonitrile, and use 1 µL of this diluted solution as an HPLC sample solution.
TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )
HPLC Labeling Reagents ( PDF 10MB )
References
PubMed Literature
Articles/Brochures
TCIMAIL
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Specifications
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