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CAS RN: 65-61-2 | Product Number: A3396

Acridine Orange Solution [for Cell Staining]

× Acridine Orange Solution [for Cell Staining]

Purity:
Synonyms:
Product Documents:
SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications & Literature

Supplemental Product Information
5ML
$38.00
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Supplemental Product Information:

Product Information
Volume : 5 mL
Product Form : 0.05 mg/mL solution

Description
Acridine orange is a cell membrane-permeant nucleic acid cell stain, which is incorporated into double-stranded DNA at a ratio of one to three base pairs. It fluoresces at 520nm (Ex. 500nm) when bound to double stranded DNA, and 650nm (Ex. 460nm) when bound to RNA or single-stranded DNA. As acridine orange is mutagenic, this product is supplied as a pre-made solution to prevent exposure during weighing.

Direction for Use
Adjust volume and staining time as necessary. Because some cells need to be fixed prior to staining, preliminary testing should be performed.
1. Remove medium from cells and wash twice with cold PBS(-).
2. Remove PBS(-) and add an appropriate volume of 1:50 Acridine Orange (Product No. A3396) in PBS(-). Allow to stain for 15 minutes.
3. Remove the staining solution and wash twice with PBS(-).
4. Observe cells with a fluorescence microscope while submerged in PBS(-).

Storage
Upon receipt the product should be stored at 4 °C, protected from light, and used within 6 months.

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Product Number A3396
Molecular Formula / Molecular Weight C__1__7H__1__9N__3·HCl = 301.82 
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 65-61-2
PubChem Substance ID 468590276
MDL Number

MFCD00012660
Specifications
Appearance Light yellow to Brown clear liquid
DNA staining test to pass test
Properties (reference)
GHS
Related Laws:
RTECS# AR7601000
Transport Information:
HS Number 3204.19.5000
Application
Cell Staining

References


Articles/Brochures

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Safety Data Sheet (SDS)
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