MTT Solution [for Cell proliferation assay] (1mL×5)

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Synonyms:

• 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium Bromide Solution (1mL×5)

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Supplemental Product Information

Size	Unit Price	Belgium	Japan*	Quantity
1SET	€65.00	1	≥60

*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).

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Product Information

• Volume/Quantity : 1mL x 5
• Product Form : 5.0 mg/mL in PBS

Description

• 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide (MTT) is a type of tetrazolium salt that changes to formazan when taken up into living cells. As formazan is a water-insoluble crystal, it can be dissolved in DMSO and the absorbance at 540 nm can be used to count the number of cells. This product consists of 5.0 mg/mL MTT dissolved in PBS. When 1/10th of the medium is added to cultured cells, the live cells are properly stained.

Storage

• Stored at -20 °C and protected from light. Avoid repeated freezing and thawing.

Direction for Use

• MTT solution can be added at any point in the cell culture. For best results, it should be added to cells in the logarithmic growth phase. Pre-determine the number of cells and seed 5,000 to 50,000 cells per well in a 96-well plate.

• 1. Seed 100 µL of cells in a 96-well plate.
  2. Incubate overnight in an incubator.
  3. Add 10 µL of MTT solution to each well.
  4. Incubate for 2-4 hours for color reaction.
  5. Remove the medium, taking care not to suck up the formazan.
  6. Add 100 µL of DMSO (Product No. D0798) and dissolve the formazan.
  7. Measure the absorbance at 540 nm.

Related Products

• R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
• D0798 Dimethyl Sulfoxide
• M3297 MTT [for Biochemical Research]