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Detergent for Biochemical Research

No.165(April 2015)
T3071
Tris Dodecyl Sulfate (1)
T3071
Tris dodecyl sulfate (1) is one of the anionic detergents which contains dodecyl sulfate. The property of 1 is different from that of sodium dodecyl sulfate (SDS). 1 has several advantages for biochemical analysis over SDS as described below.
Booth reported that, on two-dimensional electrophoresis of membrane proteins, 1 gives good resolution.1) Kubo and Takagi reported the effect of alkanolammonium dodecyl sulfates for Weber and Osborn’s polyacrylamide gel electrophoresis system.2) The abilities of the dodecyl sulfates as to the dissociation of the molecular assemblies of chlorophyll-proteins were found to decrease in the following order: 1 > triethanolammonium salt > triisopropanolammonium salt.
Depending upon antigens and antibodies, 1 gives different reactivities from SDS on immunoblotting.3) 1 was also utilized for chromatographical separation of rat tail tendon collagen from monomeric components.4)

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