Keratanase II, Hydrolytic Enzyme Specific to Keratan Sulfate

Keratan sulfate is a sulfated polysaccharide classified as a glycosaminoglycan, an essential component of proteoglycans. It is found in tissues such as the cornea of the eye, joints, and skin, playing crucial roles in these tissues. Additionally, it has been reported to be involved in muscle and lung diseases and is also recognized as a stem cell marker.¹⁾
Keratanase II is a recombinant enzyme expressed in Escherichia coli using a gene derived from Bacillus circulans, and specifically acts on keratan sulfate by hydrolyzing the β1-3 linkage between N-acetylglucosamine (GlcNAc) and galactose (Gal). GlcNAc sulfated in the 6-position is essential for enzyme recognition, and it does not have any effect on whether or not Gals are sulfated.²⁾

Keratanase II are commercialized under license from Kansai Medical University.

Related Products

K0069

Keratanase II from Bacillus circulans, Recombinant

A2968

Anti-Keratan Sulfate Monoclonal Antibody (R-10G)

Related Product Category Pages

• Glycohydrolases
• Stem Cell Research [Application Using Glyco-materials]

References

• 1) Keratan sulfate, a complex glycosaminoglycan with unique functional capability
  • B. Caterson, J. Melrose, Glycobiology 2018, 28, 182.
• 2) Purification, Characterization, and Molecular Cloning of a Novel Keratan Sulfate Hydrolase, Endo-β-N-acetylglucosaminidase, from Bacillus circulans
  • K. Yamagishi, K. Suzuki, K. Imai, H. Mochizuki, K. Morikawa, M. Kyogashima, K. Kimata, H. Watanabe, J. Biol. Chem. 2003, 278, 25766.

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