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Red Dye for Staining of Fat Cells and Lipids
Oil red O is a diazo dye which has been widely used for staining fat cell and lipids since long ago.1-3) This lysochrome (fat-soluble dye) red dye can be used for staining of neutral triglycerides and low polar lipids. Oil Red O staining is done on fresh or frozen samples, since alcohol fixation removes lipid. Its usage is simple, and requires only washing after adding the staining solution, which makes it easy to identify lipids visually. Furthermore, accumulation of lipids can be quantified by eluting the dye using isopropanol after staining and measuring the absorbance. In this way, oil red O has been used in diabetes, hepatitis and leukemia research, for instance.4,5)
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References
- 1)Adipose conversion of 3T3 cells depends on a serum factor
- 2)Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O.
- 3)Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with oil red O
- 4)Hepatocellular ballooning in NASH.
- 5)Expression of homologues for p53 and p73 in the softshell clam (Mya arenaria), a naturally-occurring model for human cancer
- 6)Oil red-O stains non-adipogenic cells: a precautionary note
