Maximum quantity allowed is 999
CAS RN: | 제품번호: T3854
TMB [for ELISA] (Ready-to-use solution)
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제품보충정보:
Product Information
Volume : 100 mL
Description
This product is supplied as a ready-to-use solution containing 3,3',5,5'-Tetramethyl -benzidine (TMB) and hydrogen peroxide for ELISA. When this product reacts with horseradish peroxidase (HRP), a blue colored soluble reaction product appears. Its absorbance can be measured at 650 nm. After terminating the reaction by adding hydrochloric acid, the reaction product becomes yellow and its absorbance can be measured at 450 nm.
Direction for Use
The following steps are for a 96-well plate format.
1. Bring TMB solution (Product No. T3854) to room temperature.
2. Gently mix the TMB solution (Product No. T3854).
3. Add 100uL of TMB solution (Product No. T3854) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100uL of 1 N HCl (Product No. H1202) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 450 nm.
Storage
The product should be stored at 4 ºC, and used within 6 months.
Precipitations sometimes arise, but they do not affect the quality.
Related Products
T3855 TMB (Ready-to-use solution) [for Western blotting]
T2573 3,3',5,5'-Tetramethylbenzidine [for Biochemical Research]
H1202 Hydrochloric Acid (1mol/L)
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제품번호 | T3854 |
물리적 상태 (20 ℃) | Liquid |
보관 조건 | Refrigerated (0-10°C) |
피해야 할 조건 | Heat Sensitive |
Appearance | Colorless to Almost colorlessclear liquid |
Peroxidase detection test | to pass test |
픽토그램 |
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신호 워드 | Danger |
위험물 및 유해 등록 | H370 : Causes damage to organs. H372 : Causes damage to organs through prolonged or repeated exposure. H227 : Combustible liquid. |
주의 사항 | P501 : Dispose of contents/ container to an approved waste disposal plant. P270 : Do not eat, drink or smoke when using this product. P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. P403 : Store in a well-ventilated place. P405 : Store locked up. |
HS 번호* | 3822.19-000 |
References
- Cold Spring Harbor Laboratory Press, New York
- E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p404, p506, p594
SDS
요청한 SDS를 사용할 수 없습니다.
번거롭게 해드려 죄송하지만 이 양식 보다 문의 해주십시오.
규격표
시험성적서, 각종 증명서
샘플 시험성적서
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분석 차트
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