Sialidase from Arthrobacter ureafaciens

×

순도/분석 방법:

동의어의:

• Neuraminidase from Arthrobacter ureafaciens
• α²-3,6,8 Sialidase from Arthrobacter ureafaciens

제품문서:

SDS | 규격표 | 시험성적서， 각종 증명서 | 분석 차트

일반 정보 | 규격치/물성치 | 안전 및 규정 | 이용 예・문헌

제품보충정보

이 페이지에 대한 설명

포장단위	가격	당일출고	2-3일 이내 출고	추가로 필요한 경우	출하 정보
1VIAL	₩267,200	10	0	문의	출하 시뮬레이션

•본 제품의 재고는 일본 본사의 재고입니다. 주문시, 5일안에 실험실까지 도착됩니다.
•본건의 원가격은 한국 대리점의 예상 판매가격입니다.자세한 정보가 필요하시면 연락해 주십시오.( SEJIN CI Co., Ltd. (한국총대리점) 전화 : 02-2655-2480 이메일 :  sales@sejinci.co.kr)
•보관 조건은 예고없이 변경 될 수 있습니다. 제품 보관 조건의 최신 자료는 홈페이지에 기재되어 있으니 양해 부탁드립니다.

대량 구매 및 견적의뢰

Product Information

• Quantity : 1.0 U per vial (10 U/mL)
• Product Form : Purified Sialidase, Liquid
• Storage buffer : 20 mM Sodium phosphate buffer, pH 7.4
• Preservative : None
• Stabilizer : None

Description

• This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.

Unit Definition

• One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (Product No. M3031) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Neu5Ac-α-4MU in 40 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.

Storage

• Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
  (For long-term use, aliquot and freeze after thawing.)

Related Products

• G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
• I1206 Sialidase (Neuraminidase) Agarose
• 
  
• Other oligosaccharide-releasing enzymes
• [Endo-type N-glycanase (ENGase)]
• A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
• E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
• G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
• 
  
• [Endo-type O-glycanase]
• A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
• 
  
• [Endo-type glycoceramidase]
• R0240 rEGCase I
• R0241 rEGCase I assisted by Activator II
• R0242 rEGCase II
• R0243 rEGCase II assisted by Activator II
• 
  
• [Endo-type glycosaminoglycanase]
• K0069 Keratanase II (for hydrolysis of Keratan sulfate)