Maximum quantity allowed is 999
CAS RN: | 제품번호: B6105
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
포장단위 | 가격 | 당일출고 | 2-3일 이내 출고 |
---|---|---|---|
10ML |
₩92,500
|
≥60 | 10 |
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•보관 조건은 예고없이 변경 될 수 있습니다. 제품 보관 조건의 최신 자료는 홈페이지에 기재되어 있으니 양해 부탁드립니다.
제품보충정보:
Product Information
Volume : 10 mLDescription
This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25 - 30 °C if precipitation occurs during storage.
Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95 - 100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (TCI Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (TCI Product No. D3647), etc.
Storage
Store at room temperature.
Related Products
A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
제품번호 | B6105 |
물리적 상태 (20 ℃) | Liquid |
보관 조건 | Room Temperature (Recommended in a cool and dark place, <15°C) |
Appearance | Green to Dark green to Dark blue clear liquid to cloudy liquid |
Functionality test | to pass test(Electrophoresis) |
비중 | 1.16 |
픽토그램 | |
신호 워드 | Warning |
위험물 및 유해 등록 | H315 : Causes skin irritation. |
주의 사항 | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves. P302 + P352 : IF ON SKIN: Wash with plenty of water. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
HS 번호* | 3822.19-000 |
References
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
- Dodecyl sulfate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.
SDS
요청한 SDS를 사용할 수 없습니다.
번거롭게 해드려 죄송하지만 이 양식 보다 문의 해주십시오.
규격표
시험성적서, 각종 증명서
샘플 시험성적서
본 제품의 샘플시험성적서는 현재 준비되어 있지 않습니다.
분석 차트
죄송합니다만 찾으시는 분석차트는 없습니다.