Phenyl Isothiocyanate [for HPLC Labeling]

The compound 1 is an HPLC labeling reagent, which has an isothiocyano group, can easily react with an amino group to form the corresponding thiourea. The resultant thiourea can be also derivatized into a phenylthiohydantoin (PTH) derivative under acidic conditions. The PTH is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 269 nm for UV detection.

Application examples:
[Amino acids, Peptides]
1.5 µmol of a sample is dissolved into 1 mL of 60% aqueous pyridine solution containing labeling reagent 1 (15 mg), and incubated at 40 °C for 1 h.After cooling to room temperature, the reaction mixture is diluted with 1 mL of water, and excess amount of 1 is removed by extraction (benzene 2 mL x 4 times). The aqueous layer is evaporated, and dried in desiccator. To the residue, 1.5 mL of mixed solution (3 N HCl and 60% AcOH, 1 : 1) is added to hydrolyzed at 40 °C for 30 min under a nitrogen atmosphere. After cooling to room temperature, the reaction mixture is diluted with 2 mL of water, and extracted with 2 mL of ethyl acetate, next 2 mL of benzene. The organic layers are combined to use it as an HPLC sample.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )