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CAS RN: | Product Number: G0622
O-Glycosidase for core1 (Endo-α and Sialidase mix)
Purity:
Synonyms:
- O-Glycosidase for core1 (Endo-α & Neuraminidase mix)
Product Documents:
Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
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1VIAL |
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9 | 0 | Contact Us |
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* The storage conditions are subject to change without notice.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
- Quantity : Endo-α 0.1 U per vial (1 U/mL)
- Sialidase 1.0 U per vial (10 U/mL)
- Product Form : Purified Endo-α and Sialidase, Liquid
- Storage buffer : 20 mM Sodium phosphate, 150 mM NaCl buffer, pH 7.4
- Preservative : None
- Stabilizer : None
Description
- Endo-α
- This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.
- Sialidase
- This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.
Unit Definition
- Endo-α
- One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No.G0375) per minute at 37°C under the condition of pH 5.0.
- Sialidase
- One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (product code: M3031) per minute at 37°C under the condition of pH 5.0.
Assay Conditions
- Endo-α
- Reaction conditions: 50 mM Sodium acetate, pH 5.0
The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer. - Sialidase
- Reaction conditions: 50 mM Sodium acetate, pH 5.0
The reaction mixture includes the enzyme and Neu5Ac-α-4MU in 40 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.
Storage
- Store at -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks. <br>(For long-term use, aliquot and freeze after thawing.)
Related Products
- I1205 Sialidase (Neuraminidase)
- I1206 Sialidase (Neuraminidase) Agarose
- Other oligosaccharide-releasing enzymes
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0241 rEGCase I assisted by Activator II
- R0242 rEGCase II
- R0243 rEGCase II assisted by Activator II
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
Product Number | G0622 |
Physical State (20 deg.C) | Liquid |
Storage Temperature | Frozen (-80°C) |
Condition to Avoid | Heat Sensitive |
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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