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CAS RN: | Product Number: B6110
2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
Shipping Information
|
|---|---|---|---|---|---|
| 25ML |
₩76,100
|
25 | 0 | Contact Us |
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* The storage conditions are subject to change without notice.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
Volume: 25 mLDescription
This product contains 4% SDS, 20% glycerol, 0.02% phenol red and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.
Note: Please use after bringing back to normal temperature.
Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 μL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No.: M1948), DL-Dithiothreitol [for Electrophoresis] (Product No.: D3647), etc.
Storage
Store at room temperature.
Related Products
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B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6105 6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0316 Glycerol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
P0102 Phenol Red Sodium Salt
S0588 Sodium Dodecyl Sulfate [for Electrophoresis]
T2516 Tris(hydroxymethyl)aminomethane [for Electrophoresis]
| Product Number | B6110 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Suitability for Electrophoresis | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.07 |
GHS
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
Red 2X Sample Buffer for Differentiating Protein Samples by Color
Figure. View when a sample using 1 is applied (arrow)
References
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
- Native SDS-PAGE: high resolution electrophoretic separation of proteins with retention of native properties including bound metal ions
Articles/Brochures
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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