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CAS RN: 59793-96-3 | Product Number: A1844
endo-α-N-Acetylgalactosaminidase (=Endo-α) Recombinant: from Bifidobacterium longum expressed in Escherichia coli
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Synonyms:
- Endo-α Recombinant: from Bifidobacterium longum expressed in Escherichia coli
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days |
|---|---|---|---|
| 1VIAL |
₩321,900
|
13 | Contact Us |
* Please contact our distributor of SEJIN CI Co., Ltd.
if you would like to purchase TCI products. The above prices do not include freight cost, customs charge and other charges to the destination. SEJIN CI Co., Ltd. (Phone: 02-2655-2480 / email:
sales@sejinci.co.kr)
* The storage conditions are subject to change without notice.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
- Quantity : 100 mU per vial
- Product Form : Purified Endo-α, Lyophilized powder
- Preservative : None
- Stabilizer : None
Description
- This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.
Unit Definition
- One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No. G0375) per minute at 37°C under the condition of pH 5.0.
Assay Conditions
- Reaction conditions: 50 mM Sodium acetate, pH 5.0
The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer.
Storage
- Store at -80°C in its lyophilized powder form. Use promptly after dissolving in buffer.
Related Products
- I1205 Sialidase (Neuraminidase)
- I1206 Sialidase (Neuraminidase) Agarose
- G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
- Other oligosaccharide-releasing enzymes
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0241 rEGCase I assisted by Activator II
- R0242 rEGCase II
- R0243 rEGCase II assisted by Activator II
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
| Product Number | A1844 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
| CAS RN | 59793-96-3 |
| Enzyme Commission Number | 3.2.1.97 |
| MDL Number | MFCD00131003 |
Specifications
| Appearance | White powder to crystal |
| Titer | 80 ～ 120 mUnit/1 Vial |
Properties (reference)
GHS
Related Laws:
Transport Information:
| H.S.code* | 3507.90-000 |
Application
Argatroban: A Direct Thrombin Inhibitor Act as an Anticoagulant
References
- K. Fujita, F. Oura, N. Nagamine, T. Katayama, J. Hiratake, K. Sakata, H. Kumagai, K. Yamamoto, J. Biol. Chem. 2005, 280, 37415.
- H. Ashida, K. Yamamoto, T. Murata, T. Usui, H. Kumagai, Arch. Biochem. Biophys. 2000, 373, 394.
- T. Katayama, K. Fujita, K. Yamamoto, J. Biosci. Bioeng. 2005, 99, 457.
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