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CAS RN: | Product Number: W0024

Western Blot Stripping Buffer [for Biochemical Research]

× Western Blot Stripping Buffer [for Biochemical Research]

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Priority Assessment Chemical Substance
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Supplemental Product Information
250ML
¥6,500
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Product Information

  • Volume : 250 mL

Description

  • Western Blot Stripping Buffer is a reagent used for removing antibodies from membranes that detects chemiluminescence in Western blotting. After chemiluminescent detection, membranes treated with Stripping Buffer can be reused for antibody concentration studies or for re-detection with a different primary antibody. The Stripping Buffer is useful for saving samples and shortening the experiment time.
    Note: Dissolve the precipitation at 25-30 °C, if precipitation occurs during storage. The Stripping Buffer is acidic and corrosive. Please wear protective equipment when using it. Metal tweezers and trays should not be used with the Stripping Buffer.

Storage

  • Store at room temperature.

Direction for Use

    1. After detecting chemiluminescence, wash the membrane twice for 10 minutes with TBS-T or PBS-T buffer.
    2. Incubate the membrane with the Stripping Buffer for 30 minutes at room temperature. Use a sufficient volume of the Stripping Buffer to ensure that the membrane is completely submerged.
      Note: Depending on the affinity between the antigen and the antibody, the antibody may not be completely removed from the membrane.
    3. Wash the membrane three times for 10 minutes each with TBS-T or PBS-T buffer.
    4. Perform blocking and antigen-antibody reaction again.

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Product Number W0024
Physical State (20 deg.C) Liquid
Storage Temperature Room Temperature (Recommended in a cool and dark place, <15°C)
Specifications
Appearance Colorless to Almost colorlessclear liquid
Functionality test to pass test
Properties (reference)
Specific Gravity (20/20) 1.00
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H373 : May cause damage to organs through prolonged or repeated exposure.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P260 : Do not breathe dust/ fume/ gas/ mist/ vapors/ spray.
P314 : Get medical advice/ attention if you feel unwell.
Related Laws:
Chemical Substance Law (ENCS) Priority Assessment Chemical Substance
PRTR Law (New Specific Chemical) Class 1 Designated Chemical Substances
Transport Information:
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