rEGCase II

×

Purity:

Synonyms:

• Recombinant Endoglycoceramidase II
• Recombinant - Rhodococcus sp. EGCase II expressed in Rhodococcus erythropolis

Product Documents:

SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications & Literature

Supplemental Product Information

Size	Unit Price	Saitama (Kawaguchi)	Hyogo (Amagasaki)	Stock in other WH	Shipping Information
1VIAL	¥27,000	5	1	Contact Us	Shipping Simulation

Request Bulk Quote

* Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock In Other WH: Shipment in about 2-3 Business Days from Saitama warehouse. Please contact us if you need further information.
* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.

Product Information

• Quantity : 100 mU per vial (2 mU/μL)
• Product Form : Purified recombinant EGCase II, Liquid
• Storage buffer : 20 mM sodium phosphate, 50 mM NaCl buffer, pH 7.0
• Optimum pH : pH 5.5
• Preservative : None
• Stabilizer : None

Description

• Endoglycoceramidase II (EGCase II) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under conditions requiring a detergent or a cofactor Activator II. The EGCase II gene is derived from Rhodococcus sp. and its recombinant protein is expressed in Rhodococcus erythropolis.

Unit Definition

• One unit (U) of EGCase II is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH 5.5.

Assay Conditions

• Reaction conditions: 50 mM sodium acetate containing 0.4% Triton X-100, pH 5.5
  The reaction mixture includes the enzyme and 100 nmol of GM1 in 50 μL. The mixture is incubated at 37°C for 15 minutes. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see the following reference).
• [Reference]
  Determination of Glucose by a Modification of Somogyi-Nelson Method
  C. Hatanaka, Y. Kobara, Agric. Biol. Chem. 1980, 44, 2943.

Storage

• Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.

Related Products

• G0483 Ganglioside GM₁
• P1775 TRITON™ X-100
• 
  
• Other oligosaccharide-releasing enzymes
• [Endo-type glycoceramidase]
• R0240 rEGCase I
• R0241 rEGCase I assisted by Activator II
• R0243 rEGCase II assisted by Activator II
• 
  
• [Endo-type N-glycanase (ENGase)]
• A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
• E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
• G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
• 
  
• [Endo-type O-glycanase]
• A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
• G0622 O-Glycosidase for core1 (for hydrolysis of sialylated core1 O-glycan epitope)
• 
  
• [Endo-type glycosaminoglycanase]
• K0069 Keratanase II (for hydrolysis of Keratan sulfate)