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CAS RN: | Product Number: C3708
CUBIC-HV™1 3D immunostaining kit
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| Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
Shipping Information
|
|---|---|---|---|---|---|
| 1KIT |
¥98,000
|
Contact Us | Contact Us | Contact Us |
* Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock In Other WH: Shipment in about 2-3 Business Days from Saitama warehouse. Please contact us
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
This kit contains buffers and anti-NeuN antibody (positive control) for 3D nuclear staining of CUBIC-cleared tissues.
The difference between CUBIC-HV™1 3D immunostaining kit (Product No. C3708) and CUBIC-HV™1 3D immunostaining kit (Casein separately) (Product No. C3717):
CUBIC-HV™1 3D immunostaining kit (Product No. C3708) includes a buffer with casein, which can be used as soon as it is arrived. The buffer with casein should be used as soon as possible.
CUBIC-HV™1 3D immunostaining kit (Casein separately) (Product No. C3717) includes a buffer without casein and casein is in the kit separately. The casein should be mixed to the buffer just before use and after mixing, the buffer should be used as soon as possible. It takes about 2 hours to solve the casein.
Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html
Constituents in kit (suitable for a brain of an adult mouse)
- CUBIC-HV™1 3D immunostaining buffer (2x) (for 10 tests)
- CUBIC-HV™1 3D immunostaining wash buffer (1x) (for 10 tests)
- CUBIC-HV™1 3D immunostaining additive (10x) (for 10 tests)
- Anti-NeuN Mouse IgG1 antibody (1mg/mL) (for 2 tests)
- 10x 15 mL tube
Process outline
====Delipidation====
Perfusion fixation and dissection of the mouse brain
Post-fix <24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3-7 days
Wash (PBS) 2 h x3
====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709) ====
Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3
==== Optional process; Enzyme reaction ====
====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====
Primary antibody (anti NeuN antibody in C3708 kit can be used): 5 µg/whole brain
Secondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week
Reaction in the same buffer at 4°C, 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3
====Fix and RI matching====
Post-fix (in 1% Formaldehyde Solution), 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740), <3 days
Microscopic observation
Related Products
T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]T3983 Tissue-Clearing Reagent CUBIC-R+(N) [for RI matching]
T3741 Tissue-Clearing Reagent CUBIC-R+(M) [for RI matching]
C3708 CUBIC-HV™1 3D nuclear staining kit
C3717 CUBIC-HV™1 3D immunostaining kit (Casein separately)
F0622 37% Formaldehyde Solution
H0396 HEPES
| Product Number | C3708 |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
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Application
3D staining and imaging for large specimens
References
- Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
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