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CAS RN: 221263-97-4 | Product Number: A5573
NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
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![NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]")
Purity: >95.0%(HPLC)
Synonyms:
- 4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitrobenzofurazan
- 4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole
Product Documents:
| Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
Shipping Information
|
|---|---|---|---|---|---|
| 100MG |
¥27,500
|
7 | 1 | It can be shipped in about 3-4 weeks after ordering |
* Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock In Other WH: Shipment in about 2-3 Business Days from Saitama warehouse. Please contact us
if you need further information.
* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
| Product Number | A5573 |
| Purity / Analysis Method | >95.0%(HPLC) |
| Grade | Ace |
| Molecular Formula / Molecular Weight | C__9H__1__0N__6O__4 = 266.22 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | Refrigerated (0-10°C) |
| Store Under Inert Gas | Store under inert gas |
| Condition to Avoid | Moisture Sensitive,Heat Sensitive |
| Packaging and Container | 100MG-Glass Bottle with Plastic Insert (View image) |
| CAS RN | 221263-97-4 |
| PubChem Substance ID | 87563190 |
| MDL Number | MFCD02093433 |
Specifications
| Appearance | Orange to Brown powder to crystal |
| Purity(HPLC) | min. 95.0 area% |
| Elemental analysis(Nitrogen) | 30.00 to 33.15 % |
| Effect test of HPLC derivatization | Effective as labeling agent fo Ibuprofen |
| NMR | confirm to structure |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
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Application
HPLC Labeling Reagent for Carboxylic Acids
The compound 1 an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carboxyl group to form the corresponding carbohydrazide in the presence of a condensing agent. The resultant carbohydrazide is stable for at least one week at 4 °C. The carbohydrazide derivatives can be analyzed by reversed phase HPLC, and an excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 475 nm and 530 nm, respectively. [The detection limit = 2~4 fmol (S/N = 3)]
Application example:
Add 50 µL of carboxylic acid / DMF solution, 50 µL of 1.0 M EDC aqueous solution, 50 µL of 20% pyridine aqueous solution and 20 mM HPLC labeling reagent 1 / DMF solution to a vessel, and incubate the mixture at room temperature for 2 h. Dilute this reactant mixture 10x with the mobile phase solution, and use 1 µL of this diluted solution as an HPLC sample solution.
Add 50 µL of carboxylic acid / DMF solution, 50 µL of 1.0 M EDC aqueous solution, 50 µL of 20% pyridine aqueous solution and 20 mM HPLC labeling reagent 1 / DMF solution to a vessel, and incubate the mixture at room temperature for 2 h. Dilute this reactant mixture 10x with the mobile phase solution, and use 1 µL of this diluted solution as an HPLC sample solution.
TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )
HPLC Labeling Reagents ( PDF 10MB )
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