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CAS RN: | Product Number: L0466
Linear PEI HCl [1mg/mL in PBS(-)] [for Transfection]
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Purity:
Synonyms:
- Linear Polyethyleneimine Hydrochloride [1mg/mL in PBS(-)]
Product Documents:
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 1ML |
₹3,200.00
|
Contact Us | 3 |
|
| 10ML |
₹15,800.00
|
Contact Us | 12 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Description
- This product is supplied as a pH-adjusted, 1 mg/mL stock solution of the linear form of the cationic polymer polyethylenimine (PEI) to be used for the transfection of nucleic acids into living cells.
Directions for Use
-
-
- Prepare two aliquots of OPTI-MEM®. Each should be equivalent to 1/4 the regular culture maintenance volume of your cells.
- Into one aliquot, mix an appropriate amount of L0466 (*). Into the other aliquot, mix an appropriate amount of DNA (*).
- Combine the two aliquots from step 2 and incubate at 37°C for 20 minutes.
- Add an equivalent volume of regular culture medium to the solution from step 3.
- Aspirate medium from cells and replace with the medium from step 4.
- Allow transfection to proceed for between 6 and 24 hours.
- Replace medium and incubate until protein expression is detected.
- Use the cells for downstream applications.
- Create a standard curve to calculate the MDA concentration in the sample.
†Opti-MEM is a registered trademark of Life Technologies Corporation. -
Storage
- Store at 4°C. Use within 6 months of receipt.
| Product Number | L0466 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Transfection Efficiency Test | to pass test |
| Cytotoxicity Test | to pass test |
Properties (reference)
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