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Nucleobase-specific Probes [RNA Structure Analysis]
Numerous compounds are known as base-specific probes, including DMS, a small molecule probe that was first used for RNA structural analysis, CMCT, and Kethoxal. For obtaining structural information for all nucleotides using base-specific probes, multiple reagents are required.
| Probe | Primary Modification Sites | Product Number |
|---|---|---|
| DMS | Guanine N7, Adenine N1, Cytosine N3 | |
| DEPC | Adenine N7 | |
| CMCT | Guanine N1, Uracil N3 | |
| Kethoxal | Guanine N1 and N4 | - |
| DMAS-Cl | Guanine N2 | - |
| Naz | Guanine C8, Adenine C8 |
[Abbreviations]
DMS: Dimethyl Sulfate; DEPC: Diethyl Pyrocarbonate, CMCT: 1-Cyclohexyl-3-(2-morpholinoethyl)carbodiimide Metho-p-toluenesulfonate; DMAS-Cl: N,N-(Dimethylamino)dimethylchlorosilane; Naz: Nicotinoyl Azide
DMS: Dimethyl Sulfate; DEPC: Diethyl Pyrocarbonate, CMCT: 1-Cyclohexyl-3-(2-morpholinoethyl)carbodiimide Metho-p-toluenesulfonate; DMAS-Cl: N,N-(Dimethylamino)dimethylchlorosilane; Naz: Nicotinoyl Azide
Naz (Nicotinoyl Azide) is used to study the solvent accessibility of purine nucleobases in RNA with the RNA structural analysis method LASER (Light Ativated Structural Examination of RNA).
References
- Probing the structure of RNAs in solution
- Chemical probes for higher-order structure in RNA
- DMS footprinting of structured RNAs and RNA–protein complexes
- Light-activated chemical probing of nucleobase solvent accessibility inside cells
- In Vitro Methods to Decipher the Structure of Viral RNA Genomes
![1-Cyclohexyl-3-(2-morpholinoethyl)carbodiimide Metho-p-toluenesulfonate [for Peptide Synthesis]](/medias/C0793.jpg?context=bWFzdGVyfHJvb3R8MzUxNTF8aW1hZ2UvanBlZ3xhREZsTDJobVpDODRPVEk1TXpReE5qQXpPRGN3TDBNd056a3pMbXB3Wnd8MDM1ZGFlYzMzM2Y0YzYzNWVlNmFkNDAxZGY1MTM0Y2Q4NGY0NjRmNjIxYWMwODAzYTVhOTFkZDJjMDVhYjZhMg "1-Cyclohexyl-3-(2-morpholinoethyl)carbodiimide Metho-p-toluenesulfonate [for Peptide Synthesis]")
| Product Number | C0793 |
| CAS RN | 2491-17-0 |
| Purity / Analysis Method: | >96.0%(T)(N) |
| Product Number | N1268 |
| CAS RN | 4013-72-3 |
| Purity / Analysis Method: | >95.0%(HPLC) |
| Product Number | O0103 |
| CAS RN | 1609-47-8 |
| Purity / Analysis Method: | >95%(T) |
Product Number: C0793 |
Purity / Analysis Method:
>96.0%(T)(N)
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