Published TCIMAIL newest issue No.197
Maximum quantity allowed is 999
Please select the quantity
CAS RN: | Product Number: I1265
Intracellular Reactive Oxygen Species (ROS) Detection Assay Kit
×
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* |
|---|---|---|---|
| 1KIT |
€162.00
|
Contact Us | 28 |
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
- Size : 96 tests
Description
- Reactive Oxygen Species (ROS) are mainly produced by mitochondria, regulating processes necessary for cell proliferation, including in cancer cells. Cell death, such as apoptosis and ferroptosis, is associated with an increased intracellular ROS level.1) DCFH-DA is enzymatically deacetylated and hydrolyzed to produce DCFH, which penetrates the cell. Intracellular DCFH is then oxidized to DCF by reactive oxygen species (e.g., OH, O2-, H2O2, etc.), resulting in a strong fluorophoric response that turns the solution greenish-yellow in color.2) This kit is designed to directly visualize the real-time production of activated oxygen species in live cells using fluorescence microscopy equipped with a standard green filter set (450-490 nm / 500-550 nm), or flow cytometry equipped with a blue laser (488 nm / 520 nm).
Components
- 1000X Reaction Dye (DCFH-DA) : 10 μL
- 10X Reaction Buffer : 25 mL×2
Preparation
- 1X Reaction Buffer : Reconstitute the 10X Reaction Buffer by distilled water.
- 1X Reaction Dye : Reconstitute the 1000X Reaction Dye by 1X Reaction Buffer.
Step–by–Step Protocol
Storage
- Stock the 10X Reaction Buffer and 1X Reaction Buffer at 4 °C. Stock the 1000X Reaction dye at -20 °C. Immediately use up the 1X Reaction Dye after reconstitution.
References
- 1) Mitochondrial ROS regulation of proliferating cells
- 2) Time-dependent interactions of oxidant-sensitive fluoroprobes with inhibitors of cellular metabolism
| Product Number | I1265 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
Related Laws:
Transport Information:
Articles/Brochures
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
The requested analytical chart is not available. Sorry for the inconvenience.