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CAS RN: 1239-45-8 | Product Number: E1363
Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
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![Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]")
Purity:
Synonyms:
- 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide (0.5mg/mL in Water)
- Homidium Bromide (0.5mg/mL in Water)
Product Documents:
| Size | Unit Price | Belgium | Japan* |
|---|---|---|---|
| 10ML |
31,00 €
|
3 | ≥60 |
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
Volume : 10 mLDescription
This product contain 0.5mg/mL Ethidium Bromide and can use as nucleic acid stain solution or electrophoresis buffer solution. Each drop contains 20 µg of Ethidium Bromide, so you can easily adjust the solution as final concentration. Convenient and safe to use because of dropper bottle.
Storage
The product should be stored at 4 °C, protect from light.
Description for Use
After electrophoresis, dilute an Ethidium Bromide solution to 0.5 μg/mL with water or running buffer and incubate the gel for 15-30 min. Destaining is usually not needed. If you have to decrease background fluorescence, wash the gel in water for 15 minutes. In use of electrophoresis buffer solution, Ethidium Bromide incorporated into nucleic acid and can visualize band immediately by using UV transilluminator.
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| Product Number | E1363 |
| Molecular Formula / Molecular Weight | C__2__1H__2__0BrN__3 = 394.32 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 1239-45-8 |
| Reaxys Registry Number | 3642536 |
| PubChem Substance ID | 468591733 |
| SDBS (AIST Spectral DB) | 13171 |
| Merck Index (14) | 4731 |
| MDL Number | MFCD00011724 |
Specifications
| Appearance | Light yellow to Yellow to Orange clear liquid |
| DNA staining test | to pass test |
Properties (reference)
GHS
Related Laws:
| EC Number | 214-984-6 |
| RTECS# | SF7950000 |
Transport Information:
| HS Number | 3204200090 |
Application
DNA Staining Reagents for Agarose Gel Electrohporesis
Figure 1. Agarose gel after staining with ethidium bromide (0.5 mg/mL solution) (taken in-house) 
Figure 2. Agarose gel after staining with 10X Nucleic Acid Stain Blue (taken in-house)
References
- Agarose Gel Electrophoresis for the Separation of DNA Fragments
- Detection of DNA in Agarose Gels
- J. F. Sambrook, D. W. Russell, in Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, New York, 2001, Vol. 1, Chap. 5, 14.
Application
Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
References
-
- Cold Spring Harbor Laboratory Press, New York
- Joseph Sambrook and David W. Russell., Molecular Cloning third edition, A Laboratory Manual, 2001, 5. 14.
- Importance of Binding Affinity for the Activity of a Metallodendritic Chemical Nuclease.
- Efficient removal of ethidium bromide from aqueous solution by using DNA-loaded Fe3O4 nanoparticles
- Cold Spring Harbor Laboratory Press, New York
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