Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]

×

Purity:

Synonyms:

Product Documents:

SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications

Supplemental Product Information

Size	Unit Price	Belgium	Japan*
1KIT	€152.00	Contact Us	26

*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).

Request Bulk Quote

Product Information

• Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
• Volume : 1 kit (for 2000 cm² of membrane)

Description

• This product is a reagent for the chemiluminescent detection of HRP-labeled probes that react with proteins transferred to the membrane by Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. It allows the detection of proteins at the nanogram level.

Storage

• Store at 4 °C, protected from light. Use within 6 months.

Direction for Use

• 1. Bring Solution A and Solution B to room temperature.
  2. Prepare a blotting membrane covered with HRP-conjugated probe.
  3. Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
  4. Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm² membrane.
  5. Place the membrane with the protein blotted side up on the plastic wrap or polyethylene sheet.
  6. Pour the mixture over the surface of the membrane.
  7. Incubate the membrane with this product for 60 seconds at room temperature.
  8. Remove excess mixture using paper.
  9. Place the membrane in a new wrap or polyethylene sheet.
  10. Image the membrane using an imaging system or X-ray film.

Related Products

• G0615 Gel Negative Stain kit [for Electrophoresis]
• C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
• C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
• N1113 Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
• T3855 TMB [for Western blotting] (Ready-to-use solution)