Published TCIMAIL newest issue No.197
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Thiol Group Analysis Reagents
Thiol group analysis reagents are used to quantify thiol groups (SH groups). A representative reagent is 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), which reacts with SH groups to form a yellow product, allowing quantification by measuring absorbance. DTNB is widely used for detecting trace amounts of thiol groups in biological samples.
References
- 1) Tissue sulfhydryl groups
- 2) Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)—a reexamination
- 3) Determination of cysteine in pharmaceuticals via liquid chromatography with postcolumn derivatization
![5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]](/medias/D0944.jpg?context=bWFzdGVyfHJvb3R8MzA0NTF8aW1hZ2UvanBlZ3xhR014TDJobE15ODRPVEk1T0RBMU1EYzBORFl5TDBRd09UUTBMbXB3Wnd8ZjU5MzU3ZTUwMzZkMGU2Y2E1N2M4MzNkNDg0NjhjZjE5MmU1ZWM5Y2Y1Mjg1ZDhhM2IzOTkwMWQ4M2Q2MWU1Zg "5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]")
| Product Number | D0944 |
| CAS RN | 69-78-3 |
| Purity / Analysis Method: | >98.0%(T)(HPLC) |
Product Number: D0944 |
Purity / Analysis Method:
>98.0%(T)(HPLC)
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