Plant Tissue-Clearing Reagents
The transparency technique "TOMEI" for plant analysis was developed by Prof. Matsunaga et al. at Tokyo University of Science. This method enables the clearing of Oryza sativa, Arabidopsis thaliana and many others in just a few hours. Using this technique, deeper internal structure can be clearly observed.
For more information, please see "Rapid Transparency Reagent for Plants: TOMEI" page.
Figure 1. Cleared Oryza sativa (left) and Arabidopsis thaliana (right) by TOMEI
On the other hand, improved TOMEI (iTOMEI) is a method that specializes TOMEI for fluorescent protein observation. This method enables simply transparency and clearly detecting fluorescent protein.
For more information, please see "Plants Tissue-Clearing Reagent Suitable for Fluorescent Protein Observation: iTOMEI" page.
Figure 2. Cleared Arabidopsis thaliana by iTOMEI
(left) Non-treatment (right) iTOMEI treatment
Plants tissue clearing protocol movie for iTOMEI reagents
