Fluorescent Probe ADHP to Detect Hydrogen Peroxide

Hydrogen peroxide is an oxidative stress factor in cells, and the detection of hydrogen peroxide in cell culture supernatant is widely used as an indicator of oxidative stress. ADHP (1) is a fluorescent probe for detecting hydrogen peroxide; in the presence of HRP (horseradish peroxidase), it is oxidized by hydrogen peroxide to form resorufin, which exhibits fluorescence with an excitation maximum at 563 nm and an emission maximum at 587 nm.¹⁾ Leveraging this characteristic, 1 is also used to detect reactive oxygen species within cells.²⁾

References

• 1) Steady-State Kinetic Characterization of Substrates and Metal-Ion Specificities of the Full-Length and N-Terminally Truncated Recombinant Human Methionine Aminopeptidases (Type 2)
  • G. Yang, R. B. Kirkpatrik, T. Ho, G. F. Zhang, P. H. Liang, K. O. Johanson, D. J. Casper, M. L. Doyle, J. P. Marino Jr, S. K. Thompson, W. Chen, D. G. Tew, T. D. Meek, Biochemistry 2001, 40, 10645.
• 2) Analysis of mitochondrial generation and release of reactive oxygen species
  • G. Mattiasson, Cytometry A 2004, 62A, 89.

Related Products

H1222

Hydrogen Peroxide (35% in Water)

P3192

Peroxidase from Horseradish

R0012

Resorufin

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