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Published TCIMAIL newest issue No.197
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Reagents for Introduction of Photolabile Protecting Group
The “caging” of bioactive molecule with photolabile protecting groups has proven to be a useful tool in biochemical research. Caged compounds are bioactive substances inactivated with photolabile protecting groups1) and can be activated by UV or visible photoirradiation. Native compounds such as biotin, ATP, and others can be protected and caged with photolabile groups off of hydroxyl, amino, and phosphate group moieties. As an illustrative example, the caged compound (DEACM-ATP, 2), adenosine triphosphate protected with 7-(diethylamino)-4-(hydroxymethyl)coumarin (1), is inactive towards elongation reactions with polymerase. However, once ATP is released through photodeprotection, the elongation reactions proceed normally.2) Furthermore, research towards controlling the topical expression of activity using caged compounds has been recently reported.3)
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References
- 1)Rapid photolytic release of adenosine 5'-triphosphate from a protected analog: utilization by the sodium:potassium pump of human red blood cell ghosts
- 2)Light activation of transcription: photocaging of nucleotides for control over RNA polymerization
- 3)Rational Development of Caged-Biotin Protein-Labeling Agents and Some Applications in Live Cells
Other Reagents for Introduction of Photolabile Protecting Group
» 6-Bromo-7-hydroxy-4-(hydroxymethyl)coumarin
» 4-(Bromomethyl)-7-(diethylamino)coumarin
» 1-(2-Nitrophenyl)ethanol
» 2-(2-Nitrophenyl)propan-1-ol
» 4',5'-Dimethoxy-2'-nitroacetophenone
» 1-(6-Nitro-1,3-benzodioxol-5-yl)ethanol