Published TCIMAIL newest issue No.197
Maximum quantity allowed is 999
CAS RN: | Numéro de produit: R0231
rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
Pureté:
- rLSL-N-Biotin Conjugate
- Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N)-Biotin Conjugate
Taille | Prix unitaire | Belgique | Japon * | Quantité |
---|---|---|---|---|
1ML |
€245.00
|
Contactez-nous | 9 |
|
*Le délai de livraison pour des produits disponibles en stock en Belgique est 1 à 2 jours
*Le délai de livraison pour des produits disponibles en stock en Japon est 1 à 2 semaines (sauf des produits réglementés et des envois avec de la glace carbonique)
Informations supplémentaires sur le produit:
Product Information
Product code: R0231
Product name: rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant LSL-N, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >17 kDa
Preservative : None
Stabilizer: None
Description
The rLSL-N is an N-terminal domain of carbohydrate-binding protein (Lectin) derived from Laetiporus sulphureus. The rLSL-N gene product is expressed in Escherichia coli. This lectin preferably binds to [Galβ(1-4)GlcNAc, poly LacNAc] glycan epitopes.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rLSL-N-Biotin shows significant interaction with asialo human transferrin (asialo hTF) possessing [asialo] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rLSL-N-Biotin specifically binds to β(1-4)Gal-terminated hTF but not to ungalactosylated (at the non-reducing terminal) glycoproteins such as hTF, agalacto hTF and bovine RNase B.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
R0230 rPSL1a-Biotin [for Sia α(2-6)Gal]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate
Numéro de produit | R0231 |
Etat physique (20 ° C) | Liquid |
Condition de stockage | Frozen (-20°C) |
Condition à éviter | Heat Sensitive |
Appearance | Colorless to Almost colorless clear liquid |
Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
Molecular weight | 10 to 25 kDa |
Suitability for use in ELISA tests | min. 1.0 (10 micro g/mL, OD450) |
N ° SH (import / export) (TCI-E) | 3504009000 |
References
- Molecular cloning, expression, and characterization of novel hemolytic lectins from the mushroom Laetiporus sulphureus, which show homology to bacterial toxins
- Structural analysis of the Laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars
- High-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile β-trefoil lectin domain from the mushroom Laetiporus sulphureus
Articles / Brochures
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