Linear PEI HCl [1mg/mL in PBS(-)] [for Transfection]

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Synonymes

• Linear Polyethyleneimine Hydrochloride [1mg/mL in PBS(-)]

Documents de produit:

SDS | Spécifications | CoA et autres Certificats | Graphiques analytiques

Informations générales | Spécifications et Propriétés | Sécurité et Réglementaire | Applications & Litterature

Informations supplémentaires sur le produit

Taille	Prix ​​unitaire	Belgique	Japon *	Quantité
1ML	€30.00	Contactez-nous	Contactez-nous
10ML	€210.00	Contactez-nous	10

*Le délai de livraison pour des produits disponibles en stock en Belgique est 1 à 2 jours
*Le délai de livraison pour des produits disponibles en stock en Japon est 1 à 2 semaines (sauf des produits réglementés et des envois avec de la glace carbonique)

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Description

• This product is supplied as a pH-adjusted, 1 mg/mL stock solution of the linear form of the cationic polymer polyethylenimine (PEI) to be used for the transfection of nucleic acids into living cells.

Directions for Use

• • 1. Prepare two aliquots of OPTI-MEM®. Each should be equivalent to 1/4 the regular culture maintenance volume of your cells.
    2. Into one aliquot, mix an appropriate amount of L0466 (*). Into the other aliquot, mix an appropriate amount of DNA (*).
    3. Combine the two aliquots from step 2 and incubate at 37°C for 20 minutes.
    4. Add an equivalent volume of regular culture medium to the solution from step 3.
    5. Aspirate medium from cells and replace with the medium from step 4.
    6. Allow transfection to proceed for between 6 and 24 hours.
    7. Replace medium and incubate until protein expression is detected.
    8. Use the cells for downstream applications.
    9. Create a standard curve to calculate the MDA concentration in the sample.
  *Before running any transfection experiments, optimal transfection conditions (PEI concentration, N:P ratio) for your specific cell line must be determined. We recommend starting with final PEI concentrations ranging from 1 to 6 µg/mL, and N:P ratios of between 10 and 30.
  †Opti-MEM is a registered trademark of Life Technologies Corporation.

Storage

• Store at 4°C. Use within 6 months of receipt.