Maintenance Notice (4:30 AM - 11:00 AM December 21, 2024): This website is scheduled to be unavailable due to maintenance. We appreciate your patience and understanding.
Published TCIMAIL newest issue No.197
Maximum quantity allowed is 999
Please select the quantity
CAS RN: 41085-99-8 | Product Number: D6202
DiI Solution [for Cell Membrane Staining]
×
![]()
×
![DiI Solution [for Cell Membrane Staining]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "DiI Solution [for Cell Membrane Staining]")
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 1ML |
€250.00
|
Contact Us | 19 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
- Concentration :1 mM
- Excitation / Emission : 553 nm / 575 nm
Description
- DiI is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiI which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.
Storage
- Store at room temperature (below 15 °C is recommended) and protect from light.
Directions for Use: Staining live cells
-
- Dilute DiI solution to 5 μM with culture medium.
- Remove medium from cells and replace with 5 μM DiI-containing medium.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Directions for Use: Staining fixed cells
-
- Remove culture medium and wash cells twice with PBS.
- Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
- Dilute DiI solution to 5 μM with PBS.
- Remove 4% paraformaldehyde and add 5 μM DiI-containing PBS to cells.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Related Products
- D6263 DiD Solution [for Cell Membrane Staining]
| Product Number | D6202 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
| Condition to Avoid | Light Sensitive |
| CAS RN | 41085-99-8 |
Specifications
| Appearance | Light red to Red clear liquid |
| Cell staining test | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.04 |
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H319 : Causes serious eye irritation. H360 : May damage fertility or the unborn child. H372 : Causes damage to organs through prolonged or repeated exposure. H340 : May cause genetic defects. H335 : May cause respiratory irritation. H336 : May cause drowsiness or dizziness. H225 : Highly flammable liquid and vapour. |
| Precautionary Statements | P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P201 : Obtain special instructions before use. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. |
Related Laws:
Transport Information:
| UN Number | UN1170 |
| Class | 3 |
| Packing Group | II |
| HS Number | 3822190090 |
Articles/Brochures
TCIMAIL
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
The requested analytical chart is not available. Sorry for the inconvenience.