Measuring Relative Cell Number with Our ATP-Luciferase Assay Reagent

ATP-luciferase cell viability assay solution (1, 2) takes advantage of the firefly luciferase reaction, in which luciferase is reacted in the presence of ATP, magnesium, and molecular oxygen using the luciferase enzyme to produce light at roughly 560 nm. Given that the strength of the resulting bioluminescent signal scales linearly with ATP concentration, and that cells contain a more-or-less constant amount of ATP throughout the cell cycle, this reaction can be used to measure not only ATP concentration but also relative cell number (linear range = 20 - 10000 cells).^1,2) Due to the chemiluminescent nature of the reaction, no special filters are required. Additionally, direct application to plated cells changes the color of the medium to yellow, aiding in correct application.

References

• 1) Use of firefly luciferase in atp-related assays of biomass, enzymes, and metabolites
• • A. Lundin, Methods Enzymol. 2000, 305, 346.
• 2) The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity
• • S. P. M. Crouch, R. Kozlowski, K. J. Slater, J. Fletcher, J. Immunol. Methods 1993, 160, 81.

Related Compounds

M3353

MTT Solution [for Cell proliferation assay] (1mL×5)

R0195

Resazurin (Ready-to-use solution) [for Cell proliferation assay]

A5030

D-(-)-Luciferin [Chemiluminescence Reagent]

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