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CAS RN: | Product Number: W0023

WST-8 Reagent [for Cell Proliferation Assay]

× WST-8 Reagent [for Cell Proliferation Assay]

Purity:
Synonyms:
Product Documents:
SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications

Supplemental Product Information
1ML
50,00 €
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*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).

Supplemental Product Information:

Product Information

  • Volume : 1 mL (100 rxn)

Description

  • This product is supplied as a ready-to-use reagent for the measurement of relative cell number. Reduction of the water-soluble tetrazolium salt WST-8 by the cell membrane-permeable electron carrier 1-MePMS converts it to a highly colored form which can be detected using a plate reader fitted with a 460 nm absorbance filter. As 1-MePMS is itself reduced by intracellular NADH/NADPH, and NADH/NADPH concentration is relatively constant over the course of the cell cycle, the amount of reduced WST-8 and therefore the degree of color change can be used to determine relative cell number,

Direction for Use

    1. Prepare cell samples in a 96 well plate (do not remove medium).
    2. Apply 10 µL of W0023 to each well and incubate for up to 4 hours* at 37°C / 5% CO2.
    3. Measure the absorbance at 460 nm.
  • *Optimal incubation times should be determined for each individual cell line

Storage

  • Store at 4°C. Use within 6 months of receipt.

Product Number W0023
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Specifications
Appearance Light red to Red clear liquid
Cell Number-to-Signal Linearity Test to pass test
Properties (reference)
GHS
Related Laws:
Transport Information:
HS Number 3822190090
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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Analytical Charts
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