Published TCIMAIL newest issue No.197
Maximum quantity allowed is 999
CAS RN: | Product Number: T3940
Tissue-Clearing Reagent iTOMEI-D [for Plants]
Purity:
Size | Unit Price | Belgium | Japan* | Quantity |
---|---|---|---|---|
5ML |
€ 28,00
|
1 | 13 |
|
25ML |
€ 81,00
|
3 | ≥60 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.
Please see a brochure (PDF) of how to use.
Description
This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.
Storage
The product should be stored at 4 °C.
Directions for Use
Required reagents;
- Paraformaldehyde (PFA)
- PBS
- Stains* (option)
- Mounting solution**
==Process outline (All operations are performed at Room temperature)===============
- Tissue fixation
Reagent : 1% PFA in PBS
Time : 1hr - PBS Wash ×3
- Decoloring
Reagent : iTOMEI-D (TCI Product No. T3940)
Time : 24hr - PBS Wash ×3
- (Optional process Staining*, PBS Wash ×3)
- Clearing (RI matching)
Reagent : Mounting solution**
Time : Operation time is determined by the mounting solution. - Mounting
Reagent : Mounting solution**
* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.
** Mounting solution : Please use the suitable mounting solution for objective lens.
Related Products
P0018 Paraformaldehyde
A2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris-Base
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]
Product Number | T3940 |
Physical State (20 deg.C) | Liquid |
Storage Temperature | Refrigerated (0-10°C) |
Condition to Avoid | Heat Sensitive |
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